mouse nkx2 2 antibody  (Developmental Studies Hybridoma Bank)

Journal: BMC Developmental Biology

Article Title: Expression of Groucho/TLE proteins during pancreas development

doi: 10.1186/1471-213X-8-81

Figure Lengend Snippet: Counts of unambiguously identified Gro/TLE family members and eh1 or WRPW domain containing transcription factors in nine pancreas SAGE libraries

Article Snippet: Proteins pulled down using an anti-Tle2 (Santa Cruz) or anti-Hes1 (Santa Cruz) antibody were immunoblotted onto Immobilon-P membranes (Millipore) and detected with mouse anti-Nkx2-2 (Developmental Studies Hybridoma Bank), mouse anti-Nkx6-1 (Developmental Studies Hybridoma Bank), rabbit anti-Tle2 (Santa Cruz), or rabbit anti-His (Abcam).

Techniques:

Journal: BMC Developmental Biology

Article Title: Expression of Groucho/TLE proteins during pancreas development

doi: 10.1186/1471-213X-8-81

Figure Lengend Snippet: Classification of Gro/TLE family members and eh1 or WRPW domain containing transcription factors into the five expression domains in the developing pancreas using the GenePaint database

Article Snippet: Proteins pulled down using an anti-Tle2 (Santa Cruz) or anti-Hes1 (Santa Cruz) antibody were immunoblotted onto Immobilon-P membranes (Millipore) and detected with mouse anti-Nkx2-2 (Developmental Studies Hybridoma Bank), mouse anti-Nkx6-1 (Developmental Studies Hybridoma Bank), rabbit anti-Tle2 (Santa Cruz), or rabbit anti-His (Abcam).

Techniques: Expressing

Journal: BMC Developmental Biology

Article Title: Expression of Groucho/TLE proteins during pancreas development

doi: 10.1186/1471-213X-8-81

Figure Lengend Snippet: Expression of Tle1, Tle2, Tle3 and Tle4, in relation to Pdx1, Nkx2-2, Insulin, Glucagon, Pancreatic polypeptide (PP), and Somatostatin expression, in the E18.5 pancreas . Saggital sections of E18.5 pancreases were analyzed for the expression of (A, E, I, M, Q, U) Tle1, (B, F, J, N, R, V) Tle2, (C, G, K, O, S, W) Tle3 or (D, H, L, P, T, X) Tle4 (green) in relation to (A, B, C, D) Pdx1, (E, F, G, H) Nkx2-2, (I, J, K, L) Insulin, (M, N, O, P) Glucagon, (Q, R, S, T) PP, or (U, V, W, X) Somatostatin expression (red). Blue arrows indicate cells that are Gro/TLE + but without co-localization, while yellow arrows indicate cells where the Gro/TLE proteins co-localize with Pdx1, Nkx2-2, Insulin, Glucagon, PP, or Somatostatin as appropriate, and green arrows indicate cells that express Pdx1, Nkx2-2, Insulin, Glucagon, PP, or Somatostatin, as appropriate, in the absence of Gro/TLE protein. Sections were photographed at ×200.

Article Snippet: Proteins pulled down using an anti-Tle2 (Santa Cruz) or anti-Hes1 (Santa Cruz) antibody were immunoblotted onto Immobilon-P membranes (Millipore) and detected with mouse anti-Nkx2-2 (Developmental Studies Hybridoma Bank), mouse anti-Nkx6-1 (Developmental Studies Hybridoma Bank), rabbit anti-Tle2 (Santa Cruz), or rabbit anti-His (Abcam).

Techniques: Expressing

Journal: BMC Developmental Biology

Article Title: Expression of Groucho/TLE proteins during pancreas development

doi: 10.1186/1471-213X-8-81

Figure Lengend Snippet: Summary of co-localization results of Tle1, 2, 3, and 4 with Pdx1, Nkx2.2, Insulin, Glucagon, Pancreatic polypeptide (PP), and Somatostatin (SS) on E18.5 embryonic pancreas tissue

Article Snippet: Proteins pulled down using an anti-Tle2 (Santa Cruz) or anti-Hes1 (Santa Cruz) antibody were immunoblotted onto Immobilon-P membranes (Millipore) and detected with mouse anti-Nkx2-2 (Developmental Studies Hybridoma Bank), mouse anti-Nkx6-1 (Developmental Studies Hybridoma Bank), rabbit anti-Tle2 (Santa Cruz), or rabbit anti-His (Abcam).

Techniques:

Journal: BMC Developmental Biology

Article Title: Expression of Groucho/TLE proteins during pancreas development

doi: 10.1186/1471-213X-8-81

Figure Lengend Snippet: Interaction of Tle2 with selected factors . Immunoprecipitations were performed by incubating 200 ug of Min6 cellular lysate with antibodies against Tle2 (+) or IgG (-). Lysate lanes containing whole cell extracts were included as a western blot control. Blots were probed with antibodies against endogenously expressed (A) Nkx2-2 or (B) Nkx6-1. (C) Immunoprecipitations using antibodies against Hes1 (+) or IgG (-) with subsequent blotting with antibodies against Tle2. This was done since the Hes1 band was obscured by the IgG heavy chain band in anti-Tle2 immunoprecpitations with subsequent anti-Hes1 blotting. In (D) an Arx cDNA containing a C-terminal 6xHis tag was transiently transfected into Min6 cells, due to the lack of endogenous Arx expression in these cells, 48 hrs prior to the IP and the resulting immunopreciptate probed with an anti-6xHis antibody.

Article Snippet: Proteins pulled down using an anti-Tle2 (Santa Cruz) or anti-Hes1 (Santa Cruz) antibody were immunoblotted onto Immobilon-P membranes (Millipore) and detected with mouse anti-Nkx2-2 (Developmental Studies Hybridoma Bank), mouse anti-Nkx6-1 (Developmental Studies Hybridoma Bank), rabbit anti-Tle2 (Santa Cruz), or rabbit anti-His (Abcam).

Techniques: Western Blot, Transfection, Expressing

Journal: Diagnostic Pathology

Article Title: Detection of BCOR gene rearrangement in Ewing-like sarcoma: an important diagnostic tool

doi: 10.1186/s13000-021-01114-2

Figure Lengend Snippet: IHC characterisations in BCS Patients

Article Snippet: Immunohistochemistry was performed using antibodies to CD99 (O13, monoclonal, ready to use, Roche, Basel, Switzerland), Fli-1 (G146–22, monoclonal, 1:50; OriGene, Maryland, United States), CCNB3 (polyclonal, 1:300; Sigma-Aldrich, St. Louis, MO), BCOR (C-10, monoclonal, 1:100; OriGene, Maryland, United States), DUX4 (P4H2, monoclonal, 1:250; Thermo Fisher Scientific, Massachusetts, United States), NKX2.2 (EP336, monoclonal, 1:100; Origene, Maryland, United States), WT-1 (6F-H2, monoclonal, 1:100; Dako, Glostrup, Denmark), calretinin (polyclonal, 1:100; OriGene, Maryland, United States), MUC4 (8G7, monoclonal, 1:50; OriGene, Maryland, United States), TLE1 (UMAB253, monoclonal, 1:100; OriGene, Maryland, United States), EMA (GP1.4, monoclonal, 1:100; OriGene, Maryland, United States).

Techniques:

Journal: Diagnostic Pathology

Article Title: Detection of BCOR gene rearrangement in Ewing-like sarcoma: an important diagnostic tool

doi: 10.1186/s13000-021-01114-2

Figure Lengend Snippet: IHC characterisations in the 15 cases of Ewing-like sarcoma and 15 cases of negative controls

Article Snippet: Immunohistochemistry was performed using antibodies to CD99 (O13, monoclonal, ready to use, Roche, Basel, Switzerland), Fli-1 (G146–22, monoclonal, 1:50; OriGene, Maryland, United States), CCNB3 (polyclonal, 1:300; Sigma-Aldrich, St. Louis, MO), BCOR (C-10, monoclonal, 1:100; OriGene, Maryland, United States), DUX4 (P4H2, monoclonal, 1:250; Thermo Fisher Scientific, Massachusetts, United States), NKX2.2 (EP336, monoclonal, 1:100; Origene, Maryland, United States), WT-1 (6F-H2, monoclonal, 1:100; Dako, Glostrup, Denmark), calretinin (polyclonal, 1:100; OriGene, Maryland, United States), MUC4 (8G7, monoclonal, 1:50; OriGene, Maryland, United States), TLE1 (UMAB253, monoclonal, 1:100; OriGene, Maryland, United States), EMA (GP1.4, monoclonal, 1:100; OriGene, Maryland, United States).

Techniques: